Prepare an empty USB whose capacity should not be smaller than 4GB (for Windows 10 32bit) or 8GB (for 64GB). If you intend to install Windows 10 on SSD or HDD from USB drive, you can make a bootable USB drive from ISO via Diskpart utility. ▶ Create bootable Windows USB from ISO for installing Windows
PS.: It is supposed that you have downloaded Windows 10 ISO file from Microsoft and mounted the ISO file. You can choose a proper way to make a bootable USB drive of Windows 10 from ISO based on your specific demand: How to: create Windows 10 bootable USB from ISO for two purposes Get a portable Windows 10 environment: It’s available to create a Windows 10 To Go disk with a USB drive from ISO, and then you can use the familiar Windows 10 environment even when you are in different places.
Generally, there are two reasons for you to make bootable Windows 10 USB from ISO file. Why create Windows 10 bootable USB from ISO?